Summary The outbreak and spread of SARS-CoV-2 (severe acute respiratory syndrome-coronavirus-2) is a current global health emergency, and effective prophylactic vaccines are needed urgently. The spike glycoprotein of SARS-CoV-2 mediates entry into host cells, and thus is the target of neutralizing antibodies. Here, we show that adjuvanted protein immunization with soluble SARS-CoV-2 spike trimers, stabilized in prefusion conformation, results in potent antibody responses in mice and rhesus macaques, with neutralizing antibody titers exceeding those typically measured in SARS-CoV-2 seropositive humans by more than one order of magnitude. Neutralizing antibody responses were observed after a single dose, with exceptionally high titers achieved after boosting. A follow-up to monitor the waning of the neutralizing antibody responses in rhesus macaques demonstrated durable responses that were maintained at high and stable levels at least 4 months after boosting. These data support the development of adjuvanted SARS-CoV-2 prefusion-stabilized spike protein subunit vaccines. Graphical abstract Highlights Neutralizing antibodies are detectable after one adjuvanted spike protein immunization Boosting yields higher neutralizing titers than observed in seropositive human donors Neutralizing antibody titers remain high for at least 4 months after the last boost Immunization stimulates spike-specific memory B cells Mandolesi et al. show that immunization with adjuvanted prefusion-stabilized SARS-CoV-2 spike glycoprotein yields potent antibody responses in mice and macaques. Neutralizing antibodies are detectable after one immunization. Boosting results in exceptional potency, with neutralizing titers exceeding (>10-fold) those observed in seropositive humans and remaining high during a 4-month follow-up.
【저자키워드】 SARS-CoV-2, Neutralizing antibodies, protein subunit vaccine, 【초록키워드】 Vaccine, antibody, Antibody Response, Human, spike glycoprotein, Prophylactic, immunization, Spike protein, Spread, Protein, outbreak, mice, SARS-CoV-2 spike glycoprotein, response, Antibody titer, Follow-up, seropositive, Neutralizing antibody response, boost, memory B cell, single dose, Neutralizing antibody titer, SARS-CoV-2 spike, rhesus macaques, Neutralizing titer, macaques, rhesus macaque, subunit vaccines, host cells, Support, health emergency, Abstract, MONITOR, prefusion conformation, effective, Trimers, detectable, demonstrated, magnitude, stimulate, exceptional, human donor, 【제목키워드】 vaccination, mice, Neutralizing antibody response, rhesus macaque, subunit, SARS-CoV-2 protein, elicit,