World Health Organization (WHO) has prioritized the infectious emerging diseases such as Coronavirus Disease (COVID-19) in terms of research and development of effective tests, vaccines, antivirals, and other treatments. Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2), the etiological causative agent of COVID-19, is a virus belonging to risk group 3 that requires Biosafety Level (BSL)-3 laboratories and the corresponding facilities for handling. An alternative to these BSL-3/-4 laboratories is to use a pseudotyped virus that can be handled in a BSL-2 laboratory for study purposes. Recombinant Vesicular Stomatitis Virus (VSV) can be generated with complementary DNA from complete negative-stranded genomic RNA, with deleted G glycoprotein and, instead, incorporation of other fusion protein, like SARS-CoV-2 Spike (S protein). Accordingly, it is called pseudotyped VSV-SARS-CoV-2 S. In this review, we have described the generation of pseudotyped VSV with a focus on the optimization and application of pseudotyped VSV-SARS-CoV-2 S. The application of this pseudovirus has been addressed by its use in neutralizing antibody assays in order to evaluate a new vaccine, emergent SARS-CoV-2 variants (delta and omicron), and approved vaccine efficacy against variants of concern as well as in viral fusion-focused treatment analysis that can be performed under BSL-2 conditions.
【저자키워드】 SARS-CoV-2, pseudovirus, glycoprotein, Pseudotyped viruses, vesicular stomatitis virus, Biosafety Level 3, ppVSVΔG-SARS-CoV-2 S, 【초록키워드】 COVID-19, Treatment, Efficacy, Vaccine, Vaccines, S protein, spike, antivirals, SARS-CoV-2 variant, variants of concern, risk, virus, Laboratory, DNA, Pseudotyped virus, VSV, Research, emerging disease, WHO, fusion protein, respiratory, Analysis, recombinant, genomic RNA, complementary, World Health Organization, other treatments, handling, BSL-2 laboratory, pseudotyped, G glycoprotein, Complete, effective, BSL-2, vesicular, described, performed, evaluate, approved, in viral, etiological, deleted, conditions, addressed, Level, neutralizing antibody assay, 【제목키워드】 spike, Therapeutics, respiratory, vesicular, Against,