SARS-CoV-2 enters host cells when the viral spike protein is cleaved by transmembrane protease serine 2 (TMPRSS2) after binding to the host angiotensin-converting enzyme 2 (ACE2). Since ACE2 and TMPRSS2 are expressed in the tongue and gingival mucosa, the oral cavity is a potential entry point for SARS-CoV-2. This study evaluated the inhibitory effects of general ingredients of toothpastes and mouthwashes on the spike protein-ACE2 interaction and the TMPRSS2 protease activity using an in vitro assay. Both assays detected inhibitory effects of sodium tetradecene sulfonate, sodium N-lauroyl-N-methyltaurate, sodium N-lauroylsarcosinate, sodium dodecyl sulfate, and copper gluconate. Molecular docking simulations suggested that these ingredients could bind to inhibitor-binding site of ACE2. Furthermore, tranexamic acid exerted inhibitory effects on TMPRSS2 protease activity. Our findings suggest that these toothpaste and mouthwash ingredients could help prevent SARS-CoV-2 infection.
【저자키워드】 TMPRSS2, 【초록키워드】 SARS-CoV-2, ACE2, sodium, SARS-COV-2 infection, docking, protease, in vitro, angiotensin-converting enzyme 2, Spike protein, Viral, mouthwashes, ingredient, transmembrane protease serine 2, copper, tongue, binding, Inhibitory effects, Interaction, angiotensin, oral cavity, mucosa, host cells, host cell, sulfate, viral spike protein, Serine, sodium dodecyl sulfate, sodium N-lauroylsarcosinate, tranexamic acid, copper gluconate, inhibitory effect, help, transmembrane, TMPRSS2 protease, serine 2, Host, Prevent, evaluated, expressed, suggested, cleaved, mouthwashe, 【제목키워드】 ACE2, in vitro, ingredient, Protease activity, Interaction, inhibitory effect, the SARS-CoV-2,