Since December 2019, a novel coronavirus (SARS-CoV-2) has resulted in a global pandemic of coronavirus disease (COVID-19). Although viral nucleic acid test (NAT) has been applied predominantly to detect SARS-CoV-2 RNA for confirmation diagnosis of COVID-19, an urgent need for alternative, rapid, and sensitive immunoassays is required for primary screening of virus. In this study, we developed a smartphone-based nanozyme-linked immunosorbent assay (SP-NLISA) for detecting the specific nucleocapsid phosphoprotein (NP) of SARS-CoV-2 in 37 serum samples from 20 COVID-19 patients who were diagnosed by NAT previously. By using SP-NLISA, 28/37 (75.7%) serum samples were detected for NP antigens and no cross-reactivity with blood donors’ control samples collected from different areas of China. In a control assay using the conventional enzyme-linked immunosorbent assay (ELISA), only 7/37 (18.91%) serum samples were detected for NP antigens and no cross-reactivity with control samples. SP-NLISA could be used for rapid detection of SARS-CoV-2 NP antigen in primary screening of SARS-CoV-2 infected individuals.
【저자키워드】 SARS-CoV-2, Nucleocapsid phosphoprotein, Smartphone, Pt@AuNPs, POCT device, 【초록키워드】 COVID-19, coronavirus disease, virus, ELISA, Novel coronavirus, Antigen, global pandemic, cross-reactivity, China, immunoassay, nucleocapsid, SARS-CoV-2 RNA, Blood, COVID-19 patient, SARS-CoV-2 infected individuals, viral nucleic acid, serum sample, enzyme-linked immunosorbent, control sample, detect, collected, diagnosed, required, applied, immunosorbent, diagnosis of COVID-19, 【제목키워드】 detection, development, Phosphoprotein,