We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and reduced the PCR cycle of denaturation from 10 to 1 s and extension from 30 to 1 s. A cohort of 60 nasopharyngeal swab samples testing showed that the assay had a clinical sensitivity of 100% and a clinical specificity of 100%.
All Keywords
【저자키워드】 COVID-19, Nasopharyngeal swab, SARS-CoV-2 detection, ultrafast, one-step RT-qPCR assay, 【초록키워드】 RT-qPCR, Cohort, clinical sensitivity, clinical specificity, extension, nasopharyngeal swab sample, Bio-rad, denaturation, PCR cycle, significantly, reduced, reduce, 【제목키워드】 SARS-CoV-2, detection, reaction, chain,
【저자키워드】 COVID-19, Nasopharyngeal swab, SARS-CoV-2 detection, ultrafast, one-step RT-qPCR assay, 【초록키워드】 RT-qPCR, Cohort, clinical sensitivity, clinical specificity, extension, nasopharyngeal swab sample, Bio-rad, denaturation, PCR cycle, significantly, reduced, reduce, 【제목키워드】 SARS-CoV-2, detection, reaction, chain,