Background Middle East respiratory syndrome coronavirus (MERS-CoV) remains a major concern for global public health. Dromedaries are the source of human zoonotic infection. MERS-CoV is enzootic among dromedaries on the Arabian Peninsula, the Middle East and in Africa. Over 70% of infected dromedaries are found in Africa. However, all known zoonotic cases of MERS have occurred in the Arabian Peninsula with none being reported in Africa. Aim We aimed to investigate serological evidence of MERS-CoV infection in humans living in camel-herding areas in Morocco to provide insights on whether zoonotic transmission is taking place. Methods We carried out a cross sectional seroprevalence study from November 2017 through January 2018. We adapted a generic World Health Organization MERS-CoV questionnaire and protocol to assess demographic and risk factors of infection among a presumed high-risk population. ELISA, MERS-CoV spike pseudoparticle neutralisation tests (ppNT) and plaque neutralisation tests (PRNT) were used to assess MERS-CoV seropositivity. Results Serum samples were collected from camel slaughterhouse workers (n = 137), camel herders (n = 156) and individuals of the general population without occupational contact with camels but living in camel herding areas (n = 186). MERS-CoV neutralising antibodies with ≥ 90% reduction of plaque numbers were detected in two (1.5%) slaughterhouse workers, none of the camel herders and one individual from the general population (0.5%). Conclusions This study provides evidence of zoonotic transmission of MERS-CoV in Morocco in people who have direct or indirect exposure to dromedary camels.
【저자키워드】 Transmission, MERS-CoV, zoonosis, Middle East respiratory syndrome Coronavirus, Morocco, dromedaries, 【초록키워드】 protocol, Human, Infection, MERS, risk factor, ELISA, neutralising antibody, zoonotic, cross, General population, questionnaire, PRNT, Evidence, high-risk population, Contact, Middle East, Arabian Peninsula, World Health Organization, reduction, global public health, individual, zoonotic transmission, one individual, respiratory syndrome coronavirus, MERS-CoV infection, neutralisation test, serum sample, serological evidence, over, Dromedary, Result, collected, occurred, carried, reported, provide, were used, 【제목키워드】 neutralising antibody, Middle East, respiratory syndrome coronavirus,