The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.
All Keywords
【저자키워드】 COVID-19, SARS-CoV-2, Corona virus, Infectious disease, Outbreaks, Real-time PCR, molecular testing, laboratory diagnostics, 【초록키워드】 coronavirus, Europe, diagnostic, Contact tracing, Laboratory, PCR, Germany, outbreak, real-time RT-PCR, acute respiratory syndrome, primer, probe, performed, 【제목키워드】 diagnostics, Laboratory, Germany, Rapid, novel coronavirus SARS-CoV-2,
【저자키워드】 COVID-19, SARS-CoV-2, Corona virus, Infectious disease, Outbreaks, Real-time PCR, molecular testing, laboratory diagnostics, 【초록키워드】 coronavirus, Europe, diagnostic, Contact tracing, Laboratory, PCR, Germany, outbreak, real-time RT-PCR, acute respiratory syndrome, primer, probe, performed, 【제목키워드】 diagnostics, Laboratory, Germany, Rapid, novel coronavirus SARS-CoV-2,