The World Health Organization has declared COVID-19 caused by the newly discovered SARS-CoV-2 a pandemic. Due to growing demand for reagents and/or kits to extract SARS-CoV-2 RNA for subsequent RT-qPCR diagnostics, there is a worldwide risk of shortages. With a detection sensitivity of 97.4% (95% CI: 86.2–99.9%), we describe a simple, fast, alternative workflow for molecular detection of SARS-CoV-2, where samples are simply heat-processed for 5 min at 98 °C before a commonly-used RT-qPCR procedure.
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【저자키워드】 COVID-19, SARS-CoV-2, molecular diagnostics, RT-qPCR, 【초록키워드】 pandemic, risk, diagnostics, SARS-CoV-2 RNA, molecular, Detection sensitivity, World Health Organization, reagent, caused, subsequent, 【제목키워드】 molecular, Denmark,
【저자키워드】 COVID-19, SARS-CoV-2, molecular diagnostics, RT-qPCR, 【초록키워드】 pandemic, risk, diagnostics, SARS-CoV-2 RNA, molecular, Detection sensitivity, World Health Organization, reagent, caused, subsequent, 【제목키워드】 molecular, Denmark,