The new SARS-CoV-2 virus differs from the pandemic Influenza A virus H1N1 subtype (H1N1pmd09) how it induces a pro-inflammatory response in infected patients. This study aims to evaluate the involvement of SNPs and tissue expression of IL-17A and the neutrophils recruitment in post-mortem lung samples from patients who died of severe forms of COVID-19 comparing to those who died by H1N1pdm09. Twenty lung samples from patients SARS-CoV-2 infected (COVID-19 group) and 10 lung samples from adults who died from a severe respiratory H1N1pdm09 infection (H1N1 group) were tested. The tissue expression of IL-8/IL-17A was identified by immunohistochemistry, and hematoxylin and eosin (H&E) stain slides were used for neutrophil scoring. DNA was extracted from paraffin blocks, and genotyping was done in real time-PCR for two IL17A target polymorphisms. Tissue expression increasing of IL-8/IL-17A and a higher number of neutrophils were identified in samples from the H1N1 group compared to the COVID-19 group. The distribution of genotype frequencies in the IL17A gene was not statistically significant between groups. However, the G allele (GG and GA) of rs3819025 was correlated with higher tissue expression of IL-17A in the COVID-19 group. SARS-CoV-2 virus evokes an exacerbated response of the host’s immune system but differs from that observed in the H1N1pdm09 infection since the IL-8/IL-17A tissue expression, and lung neutrophilic recruitment may be decreased. In SNP rs3819025 (G/A), the G allele may be considered a risk allele in the patients who died for COVID-19.
【저자키워드】 SARS-CoV-2, immunohistochemistry, neutrophil, influenza A virus, H1N1 subtype, interleukin-8, interleukin-17A, polymorphism, 【초록키워드】 COVID-19, Neutrophils, immunohistochemistry, pandemic, IL-17A, Influenza, neutrophil, SNPs, Infection, influenza A virus, lung, SARS-CoV-2 virus, virus, influenza A, Polymorphisms, DNA, Patient, tissue expression, Genotype, IL-8, SNP, H1N1, real time-PCR, respiratory, distribution, genotyping, expression, Frequency, Hematoxylin and eosin, infected patients, eosin, hematoxylin, COVID-19 group, exacerbated response, G allele, H1N1pdm09, host’s immune system, IL-8/IL-17A tissue expression, IL17A, neutrophil scoring, neutrophils recruitment, paraffin blocks, pro-inflammatory response, risk allele, rs3819025, severe forms of COVID-19, new SARS-CoV-2, tested, evaluate, died, the patient, form, were used, correlated, induce, groups, statistically significant, was done, G/A, H&E, H1N1pmd09, neutrophilic recruitment, post-mortem lung, 【제목키워드】 COVID-19,