SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) is a novel coronavirus causing the COVID-19 pandemic in 2020. High adaptive plasticity on the spike protein of SASR-CoV-2 enables it to transmit across different host species. In the present study, we collected 2092 high-quality genome sequences of SARS-CoV-2 from 160 regions in over 50 countries and reconstructed their phylogeny. We also analyzed the polymorphic interaction between spike protein and human ACE2 (hACE2). Phylogenetic analysis of SARS-CoV-2 suggests that SARS-CoV-2 is probably originated from a recombination event on the spike protein between a bat coronavirus and a pangolin coronavirus that endows it humans infectivity. Compared with other regions in the S gene of SARS-CoV-2, the direct-binding sites of the receptor-binding domain (RBD) is more conserved. We focused on 3,860 amino acid mutations in spike protein RBD (T333-C525) of SARS-CoV-2 and simulated their differential stability and binding affinity to hACE2 (S19-D615). The results indicate no preference for SARS-CoV-2 infectivity on people of different ethnic groups. The variants in the spike protein of SARS-CoV-2 may also be a good indicator demonstrating the transmission route of SARS-CoV-2 from its natural reservoir to human hosts.
【저자키워드】 viral infection, Computational biology and bioinformatics, 【초록키워드】 SARS-CoV-2, coronavirus, Mutation, Ethnic groups, adaptive, COVID-19 pandemic, Human, variant, Transmission, severe acute respiratory syndrome Coronavirus, variants, binding affinity, Spike protein, hACE2, Novel coronavirus, human ACE2, Phylogenetic analysis, Region, stability, Receptor-binding domain, RBD, Phylogeny, respiratory, Amino acid, Interaction, S gene, Analysis, genome sequence, natural reservoir, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, plasticity, hosts, spike protein RBD, recombination event, Host, country, analyzed, collected, conserved, the spike protein, the receptor-binding domain, reconstructed, 【제목키워드】 SARS-CoV-2, adaptive, Human, Dynamics, Spike protein, binding ability, reveal,