SARS-CoV-2 is an emerging coronavirus threatening human health and the economy worldwide. As an RNA virus, variants emerge during the pandemic and potentially influence the efficacy of the anti-viral drugs and vaccines. Eight spike variants harboring highly recurrent mutations were selected and introduced into a replication-competent recombinant VSV in place of the original G protein (rVSV-SARS-CoV-2). The resulting mutant viruses displayed similar growth curves in vitro as the wild-type virus and could be neutralized by sera from convalescent COVID-19 patients. Several variants, especially Beta strain, showed resistance to human neutralizing monoclonal antibodies targeting the receptor-binding domain (RBD). A single dose of rVSV-SARS-CoV-2 Beta variant could elicit enhanced and broad-spectrum neutralizing antibody responses in human ACE2 knock-in mice and golden Syrian hamsters, while other mutants generated antibody levels comparable to the wild-type. Therefore, our results will be of value to the development of next-generation vaccines and therapeutic antibodies.
【저자키워드】 SARS-CoV-2, immunogenicity, Mutation, spike, variant, Infectivity, VSV, antigenicity, 【초록키워드】 antibodies, Efficacy, Vaccine, coronavirus, pandemic, Vaccines, antibody, hamsters, in vitro, variants, human ACE2, Health, mice, RBD, sera, therapeutic, Beta, mutant, RNA virus, anti-viral drug, Neutralizing antibody response, neutralizing monoclonal antibody, single dose, convalescent COVID-19 patients, Growth Curve, G protein, wild-type virus, wild-type, recurrent mutation, neutralized, selected, resulting, introduced, elicit, the receptor-binding domain, comparable, mutant virus, spike variant, 【제목키워드】 Revealed,