The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected over forty million patients worldwide. Although most coronavirus disease 2019 (COVID-19) patients have a good prognosis, some develop severe illness. Markers that define disease severity or predict clinical outcome need to be urgently developed as the mortality rate in critical cases is approximately 61.5%. In the present study, we performed in-depth proteome profiling of undepleted plasma from eight COVID-19 patients. Quantitative proteomic analysis using the BoxCar method revealed that 91 out of 1222 quantified proteins were differentially expressed depending on the severity of COVID-19. Importantly, we found 76 proteins, previously not reported, which could be novel prognostic biomarker candidates. Our plasma proteome signatures captured the host response to SARS-CoV-2 infection, thereby highlighting the role of neutrophil activation, complement activation, platelet function, and T cell suppression as well as proinflammatory factors upstream and downstream of interleukin-6, interleukin-1B, and tumor necrosis factor. Consequently, this study supports the development of blood biomarkers and potential therapeutic targets to aid clinical decision-making and subsequently improve prognosis of COVID-19.
【저자키워드】 proteomics, Infectious diseases, Proteomic analysis, Prognostic markers, 【초록키워드】 COVID-19, coronavirus disease, SARS-CoV-2, Necrosis, Tumor, Coronavirus disease 2019, coronavirus, Biomarker, Prognosis, SARS-COV-2 infection, disease severity, neutrophil, interleukin-6, complement, Proteins, host response, severe acute respiratory syndrome Coronavirus, Complement activation, Clinical outcome, Protein, proinflammatory, T cell, interleukin, tumor necrosis factor, severity of COVID-19, Patient, Platelet, plasma, proteome, mortality rate, respiratory, predict, proteomic, prognostic biomarker, COVID-19 patients, Blood, Analysis, critical cases, neutrophil activation, tumor necrosis, Support, Candidates, acute respiratory syndrome, Activation, Factor, Critical case, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, potential therapeutic targets, potential therapeutic target, upstream, downstream, IMPROVE, performed, develop, reported, eight, differentially expressed, highlighting, quantified, patients worldwide, 【제목키워드】 Characteristics, proteome, Blood, COVID-19 patient, plasma protein, functional, profiling analysis,