The pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been basically under control in China since March 2020, but the import of domestic SARS-CoV-2 has begun to increase. This study reported the first case of asymptomatic SARS-CoV-2 infection imported from Spain into Sichuan Province, China, on March 11, 2020. The infected male had a body temperature of 37.5°C, normal blood oxygen saturation levels, and a computed tomography (CT) examination showed that his lungs had no shadows. However, a throat swab from the subject tested positive for SARS-CoV-2 using qPCR assay. In this study, we conducted transcriptome sequencing on respiratory throat swabs from the subject and found that the dominant SARS-CoV-2 sequence (Gene Bank ID: MW301121) was a spike protein D614G mutant strain, which is currently popular throughout world. We downloaded and analyzed SARS-CoV-2 sequences collected from cases in China and Spain for comparison and tracing purposes. After March 11, 2020, the Chinese domestic clade was naturally divided into the imported SARS-CoV-2 D614G mutant strain and evolutionarily-related similar sequences and that of sequences collected in the original Wuhan area. The sequence reported in this study was located on a small branch, far from the evolution of Wuhan sequences. As expected, the identified sequence was closely related to the evolution of the SARS-CoV-2 D614G mutant strain circulating in Spain.
【저자키워드】 SARS Coronavirus, virus classification, BLAST algorithm, biostatistics and bioinformatics, clustal analysis, 【초록키워드】 Evolution, SARS-CoV-2, coronavirus, pandemic, SARS-COV-2 infection, lung, China, Computed tomography, Asymptomatic, Wuhan, male, Spain, D614G, clade, mutant, body temperature, transcriptome sequencing, Blood oxygen saturation, acute respiratory syndrome, subject, sequence, Chinese, SARS-CoV-2 sequence, circulating, positive, throat swab, qPCR assay, dominant, SARS-CoV-2 D614G, tested, analyzed, collected, caused, reported, conducted, had no, expected, Bank, downloaded, spike protein D614G, the SARS-CoV-2, tracing purposes, 【제목키워드】 SARS-CoV-2, China, Spain,