Objectives: With the worldwide spread of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), various antibody detection kits have been developed to test for SARS-CoV-2– specific IgG, IgM, and total antibody. However, the use of different testing methods under various heat-inactivation conditions might affect the COVID-19 detection results. Methods: Seven different antibody detection kits produced by four manufacturers for detection of SARS-CoV-2 IgG, IgM, and total antibody were tested at Wuhan Huoshenshan Hospital, China. Most of the kits used the indirect immunity, capture, and double-antigen sandwich methods. The effects of various heat-inactivation conditions on SARS-CoV-2-specific IgG, IgM, and total antibody detection were analyzed for the different test methods. Results: Using the indirect immunity method, values for SARS-CoV-2 IgG antibody significantly increased and those for IgM antibody decreased with increasing temperature of heat-inactivation using indirect immunity method. However, values for SARS-CoV-2 IgM and total antibody showed no change when the capture and double-antigen sandwich methods were used. The changes in IgG and IgM antibody values with the indirect immunity method indicated that heat-inactivation could affect COVID-19 detection results obtained using this method. In particular, 18 (22.2%) SARS-CoV-2 IgM positive samples were detected as negative with heat-inactivation at 65°C for 30 min, and one (25%) IgG negative sample was detected as positive after heat-inactivation at 56°C for 60 min and 60°C for 30 min. Conclusions: Heat-inactivation could increase SARS-CoV-2 IgG antibody values, and decrease IgM antibody values, causing potential false-positive or false-negative results for COVID-19 antibody detection using the indirect immunity method. Thus, before conducting antibody testing, the testing platforms should be evaluated in accordance with the relevant requirements to ensure accurate COVID-19 detection results.
【저자키워드】 COVID-19, SARS-CoV-2, heat-inactivation, IgG and IgM antibody, indirect immunity method, 【초록키워드】 coronavirus disease, IgG, IgM, Antibody testing, coronavirus, Immunity, antibody, Spread, China, Antibody detection, SARS-CoV-2 IgG antibody, Wuhan, temperature, COVID-19 antibody, false-negative, platform, SARS-CoV-2 IgG, False-positive, SARS-CoV-2 IgM, IgG and IgM, acute respiratory syndrome, IgM antibody, SARS-CoV-2-specific IgG, positive, Huoshenshan Hospital, MOST, positive sample, Effect, Affect, decrease, negative sample, significantly increased, produced, tested, analyzed, caused, indicated, evaluated, condition, were used, changes in, 【제목키워드】 Testing, Result, Potential,