The use of high-throughput small RNA sequencing is well established as a technique to unveil the miRNAs in various tissues. The miRNA profiles are different between infected and non-infected tissues. We compare the SARS-CoV-2 positive and SARS-CoV-2 negative RNA samples extracted from human nasopharynx tissue samples to show different miRNA profiles. We explored differentially expressed miRNAs in response to SARS-CoV-2 in the RNA extracted from nasopharynx tissues of 10 SARS-CoV-2-positive and 10 SARS-CoV-2-negative patients. miRNAs were identified by small RNA sequencing, and the expression levels of selected miRNAs were validated by real-time RT-PCR. We identified 943 conserved miRNAs, likely generated through posttranscriptional modifications. The identified miRNAs were expressed in both RNA groups, NegS and PosS: miR-148a, miR-21, miR-34c, miR-34b, and miR-342. The most differentially expressed miRNA was miR-21, which is likely closely linked to the presence of SARS-CoV-2 in nasopharynx tissues. Our results contribute to further understanding the role of miRNAs in SARS-CoV-2 pathogenesis, which may be crucial for understanding disease symptom development in humans.
【저자키워드】 SARS-CoV-2, small RNA sequencing, miRNAs, real-time RT-PCR, miR-21, 【초록키워드】 miRNA, Symptom, RNA, humans, RNA sequencing, nasopharynx, disease, SARS-CoV-2 pathogenesis, patients, tissue, tissues, expression level, modifications, positive, various tissues, non-infected, miRNA profiles, selected, conserved, tissue sample, contribute, groups, were expressed, differentially expressed, RNA sample, differentially expressed miRNA, miRNA profile, the SARS-CoV-2, 【제목키워드】 Human, response, Sample,