The newly identified coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causes coronavirus disease 2019 (COVID-19) and has affected over 25 million people worldwide as of August 31, 2020. To aid in the development of diagnostic kits for rapid and sensitive detection of the virus, we evaluated a combination of polymerase chain reaction (PCR) and isothermal nucleic acid amplification techniques. Here, we compared conventional PCR and loop-mediated isothermal amplification (LAMP) methods with hybrid techniques such as polymerase chain displacement reaction (PCDR) and a newly developed PCR-LAMP method. We found that the hybrid methods demonstrated higher sensitivity and assay reaction rates than those of the classic LAMP and PCR techniques and can be used to for SARS-CoV-2 detection. The proposed methods based on the modern hybrid amplification techniques markedly improve virus detection and, therefore, can be extremely useful in the development of new diagnostic kits.
【저자키워드】 COVID-19, SARS-CoV-2, Loop-mediated isothermal amplification, polymerase chain displacement reaction, PCR-LAMP, hybrid virus detection technique, 【초록키워드】 coronavirus disease, coronavirus, diagnostic, virus, amplification, SARS-CoV-2 detection, sensitivity, PCR, virus detection, isothermal amplification, nucleic acid amplification, Combination, isothermal, acute respiratory syndrome, reaction rate, polymerase chain, IMPROVE, affected, evaluated, can be used, demonstrated, cause, 【제목키워드】 detection, RNA, PCR, sensitive, technique,