The aim of this pilot study is to evaluate if SARS-CoV-2 infection or vaccination against SARS-CoV-2 infection induce observable metabolic effects in follicular fluid of women who are following in vitro fertilization (IVF) treatments. The possible impact of coronavirus disease 2019 (COVID-19) on fertility and IVF outcome is considered. We have selected for this study: six women vaccinated against SARS-CoV-2 infection, five recovered COVID-19 patients, and we used nine healthy women as the control group. At the time of oocytes retrieval from participants in the study, follicular fluids were collected and metabolomic analysis was performed by 1 H NMR spectroscopy in combination with multivariate analysis to interpret the spectral data. The search for antibody positivity in the follicular fluid aspirates was also carried out, together with the western blotting analysis of some inflammatory proteins, interleukin-6, tumor necrosis factor α (TNFα), and the free radical scavenger superoxide dismutase 2. Higher levels of Ala and Pro together with lower levels of lipids and trimethylamine N-oxide (TMAO) were found in follicular fluids (FFs) of vaccinated women while lower levels of many metabolites were detected in FFs of recovered COVID patients. Expression level of TNF-α was significantly lower both in recovered COVID-19 patients and vaccinated women in comparison to healthy controls.
【저자키워드】 COVID-19, SARS-CoV-2, metabolomics, NMR, Follicular fluid, anti SARS-CoV-2 immunoglobulins, proinflammatory markers, 【초록키워드】 coronavirus disease, Necrosis, vaccination, antibody, SARS-COV-2 infection, interleukin-6, in vitro, outcome, NMR spectroscopy, women, metabolite, TNF-α, Combination, Recovered COVID-19 patients, Lipid, Analysis, Oocyte, control group, pilot study, participant, TNFα, healthy controls, Covid patients, significantly lower, inflammatory proteins, FIVE, recovered COVID-19 patient, metabolomic analysis, selected, collected, evaluate, carried, healthy, nine, was performed, induce, Ala, dismutase, metabolic effect, Pro, radical scavenger, trimethylamine, western blotting analysis, 【제목키워드】 in vitro, recovered,