In present investigation, AND-2-HyP- β -CYD (Andrographolide-2-Hydroxypropyl- β -cyclodextrin) complex was synthesized and characterized for antiviral and pharmacokinetic profile. The linear host-guest relation suggested synthesis of a 1:1 complex of AND with 2-HyP- β -CYD by inclusion mode. The Kc, stability constant of the two phase system of AND with 2-HyP- β -CYD computed to be 38.60 x 10 −3 M. 1 H NMR spectrum of AND indicated the presence of triplet at 6.63-ppm which was up-fielded in AND-2-HyP- β -CYD complex at 6.60-ppm (doublet) confirmed the insertion of AND in cavity of 2-HyP- β -CYD through lactone ring. AND-2-HyP-β-CYD complex exhibited the IC 50 of 0.1- μ g.mL −1 (E gene) and 0.29- μ g.mL −1 (N gene) against SARS-CoV-2 infected Vero6 cells. Moreover, a 1.5-fold increment in extent of absorption of AND was noticed post complexation. The bioavailability was estimated to be 15.87 ± 3.84% and 23.84 ± 5.46%, respectively for AND and AND-2-HyP- β -CYD complex. AND-2-HyP- β -CYD complex may be a prospective candidate for further studies to evolve as a clinically viable formulation against SARS-CoV-2. Andrographolide, Cyclodextrin, SARS-CoV-2, Pharmacokinetic, Bioavailability, Antiviral
【저자키워드】 SARS-CoV-2, Antiviral, andrographolide, Bioavailability, pharmacokinetic, cyclodextrin, 【초록키워드】 stability, cells, N gene, NMR, E gene, complex, insertion, Inclusion, indicated, clinically, linear, exhibited, characterized, suggested, 1:1, 【제목키워드】 in vitro, antiviral activity, encapsulation, Analysis,