The COVID-19 pandemic has been the most critical public health issue in modern history due to its highly infectious and deathly potential, and the limited access to massive, low-cost, and reliable testing has significantly worsened the crisis. The recovery and the vaccination of millions of people against COVID-19 have made serological tests highly relevant to identify the presence and levels of SARS-CoV-2 antibodies. Due to its advantages, microfluidic-based technologies represent an attractive alternative to the conventional testing methodologies used for these purposes. In this work, we described the development of an automated ELISA on-chip capable of detecting anti-SARS-CoV-2 antibodies in serum samples from COVID-19 patients and vaccinated individuals. The colorimetric reactions were analyzed with a microplate reader. No statistically significant differences were observed when comparing the results of our automated ELISA on-chip against the ones obtained from a traditional ELISA on a microplate. Moreover, we demonstrated that it is possible to carry out the analysis of the colorimetric reaction by performing basic image analysis of photos taken with a smartphone, which constitutes a useful alternative when lacking specialized equipment or a laboratory setting. Our automated ELISA on-chip has the potential to be used in a clinical setting and mitigates some of the burden caused by testing deficiencies.
【저자키워드】 COVID-19, antibodies, SARS-CoV-2, serology, ELISA, Spike protein, Microfluidics, on-chip, automated, 【초록키워드】 public health, serological test, vaccination, equipment, COVID-19 pandemic, Image analysis, Laboratory, anti-SARS-CoV-2 antibodies, SARS-CoV-2 antibodies, Serological tests, anti-SARS-CoV-2 antibody, Smartphone, methodology, crisis, Critical, COVID-19 patients, Analysis, COVID-19 patient, serum samples, reaction, deficiencies, statistically significant difference, clinical setting, vaccinated individuals, ChIP, serum sample, microplate reader, mitigate, described, analyzed, identify, caused, significantly, demonstrated, worsened, 【제목키워드】 detection,