Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for the coronavirus disease (COVID-19) pandemic, is sweeping the world today. This study investigates the optical detection of SARS-CoV-2, utilizing the antigen-antibody binding interactions utilizing a light source from a smart phone and a portable spectrophotometer. The proof-of-concept is shown by detecting soluble preparations of spike protein subunits from SARS-CoV-2, followed by detection of the actual binding potential of the SARS-CoV-2 proteins with their corresponding antigens. The measured binding interactions for RBD and NCP proteins with their corresponding antibodies under different conditions have been measured and analyzed. Based on these observations, a “hump or spike” in light intensity is observed when a specific molecular interaction takes place between two proteins. The optical responses could further be analyzed using the principle component analysis technique to enhance and allows precise detection of the specific target in a multi-protein mixture.
【저자키워드】 COVID-19, SARS-CoV-2, RBD, NC protein, optical detection, protein–protein interactions, 【초록키워드】 coronavirus disease, severe acute respiratory syndrome coronavirus 2, coronavirus, pandemic, antibody, Proteins, severe acute respiratory syndrome Coronavirus, virus, Spike protein, Protein, response, antigens, optical, respiratory, binding, Analysis, SARS-CoV-2 proteins, followed by, intensity, acute respiratory syndrome, subunit, acute respiratory syndrome coronavirus, proof, molecular interaction, hump, binding interaction, ENhance, shown, responsible, analyzed, condition, the SARS-CoV-2, 【제목키워드】 detection,