Summary The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is continuing to disrupt personal lives, global healthcare systems, and economies. Hence, there is an urgent need for a vaccine that prevents viral infection, transmission, and disease. Here, we present a two-component protein-based nanoparticle vaccine that displays multiple copies of the SARS-CoV-2 spike protein. Immunization studies show that this vaccine induces potent neutralizing antibody responses in mice, rabbits, and cynomolgus macaques. The vaccine-induced immunity protects macaques against a high-dose challenge, resulting in strongly reduced viral infection and replication in the upper and lower airways. These nanoparticles are a promising vaccine candidate to curtail the SARS-CoV-2 pandemic. Graphical Abstract Highlights • Two-component protein nanoparticles display multiple copies of the SARS-CoV-2 spike • Spike protein nanoparticles enhance cognate B cell activation in vitro • Vaccination induces potent neutralization in mice, rabbits, and cynomolgus macaques • Vaccination protects macaques against a high-dose SARS-CoV-2 challenge Brouwer et al. present preclinical evidence in support of a COVID-19 vaccine candidate, designed as a self-assembling two-component protein nanoparticle displaying multiple copies of the SARS-CoV-2 spike protein, which induces strong neutralizing antibody responses and protects from high-dose SARS-CoV-2 challenge.
【저자키워드】 COVID-19, antibodies, SARS-CoV-2, Vaccine, Immunity, protection, B cells, Nanoparticles, macaques, 【초록키워드】 viral infection, coronavirus, COVID-19 vaccine, pandemic, spike, neutralization, Transmission, Spike protein, B cell, Replication, Protein, mice, vaccine candidate, macaque, disease, Neutralizing antibody response, cynomolgus macaques, Evidence, cynomolgus macaque, Vaccine-induced immunity, Support, High-dose, acute respiratory syndrome, Activation, lower airways, global healthcare, Prevent, ENhance, PROTECT, resulting, reduced, induce, displaying, disrupt, curtail, the SARS-CoV-2, 【제목키워드】 SARS-COV-2 infection, macaque, PROTECT,