COVID-19 patients with acute respiratory distress syndrome (ARDS) have an immune imbalance when systemic inflammation and dysfunction of circulating T and B cells lead to a more severe disease. Using TREC/KREC analysis, we studied the level of mature naive T and B cells in peripheral blood of COVID-19 patients and its relationship with clinical and laboratory data. TREC/KREC analysis was performed by multiplex real-time quantitative PCR on a sample of 36 patients aged 45 years or younger. The reduced TREC/KREC level was observed in ARDS patients compared with non-ARDS patients, and similar results were found for the deceased patients. During days 6 to 20 of hospitalization, a higher neutrophil-to-lymphocyte ratio (NLR) was detected in ARDS patients compared with non-ARDS patients. TREC/KREC negatively correlated with NLR; the highest correlation was recorded for TREC per 100,000 cells with the coefficient of determination R 2 = 0.527. Thus, TREC/KREC analysis is a potential prognostic marker for assessing the severity and outcome in COVID-19.
【저자키워드】 COVID-19, acute respiratory distress syndrome, Neutrophil-to-lymphocyte ratio, TREC/KREC, 【초록키워드】 ARDS, Hospitalization, severity, outcome, immune, Peripheral blood, B cell, Patient, Prognostic marker, systemic inflammation, multiplex, correlation, quantitative PCR, patients, acute respiratory distress, NLR, Analysis, Deceased, severe disease, COVID-19 patient, dysfunction, laboratory data, syndrome, circulating, Cell, highest, was performed, reduced, correlated, R 2, ARDS patient, was recorded, 【제목키워드】 Level,