Containment of SARS-CoV-2 has become an urgent global issue. To overcome the problems of conventional quantitative polymerase chain reaction (qPCR) tests, we verified the usefulness of a mobile qPCR device that utilizes mouthwash to obtain a saliva sample with the aim of developing a rapid diagnostic method for SARS-CoV-2. First, we examined whether anyone could easily operate this device. Then, we examined whether RNA in the mouthwash could be detected in a short time. In addition, we investigated whether it was possible to diagnose SARS-CoV-2 infection using mouthwash obtained from COVID-19 patients undergoing hospitalization. The results revealed that all subjects were able to complete the operation properly without error. In addition, RNase P was detected in the mouthwash without pretreatment. The average detection time was 18 min, which is significantly shorter than conventional qPCR devices. Furthermore, this device detected SARS-CoV-2 in the mouthwash of a COVID-19 patient undergoing hospitalization. The above findings verified the efficacy of this diagnostic method, which had a low risk of infection, was technically simple, and provided stable results. Therefore, this method is useful for the rapid detection of SARS-CoV-2.
【저자키워드】 COVID-19, SARS-CoV-2, Saliva, Mouthwash, mobile qPCR device, 【초록키워드】 Efficacy, Hospitalization, SARS-COV-2 infection, RNA, qPCR, Quantitative, diagnose, Diagnostic method, Containment, risk of infection, COVID-19 patient, average, problem, RNase P, Complete, polymerase chain, examined, addition, investigated, provided, overcome, all subject, significantly shorter, 【제목키워드】 technology, Rapid, trace, Amount,