Since the Coronavirus disease 2019 (COVID-19) pandemic outbreak, many methods have been used to detect antigens or antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), including viral culture, nucleic acid test, and immunoassay. The shear-horizontal surface acoustic wave (SH-SAW) biosensor is a novel pathogen detection platform with the advantages of high sensitivity and short detection time. The objective of this study is to develop a SH-SAW biosensor to detect the anti-SARS-CoV-2 nucleocapsid antibody. The rabbit sera collected from rabbits on different days after SARS-CoV-2 N protein injection were evaluated by SH-SAW biosensor and enzyme-linked immunosorbent assay (ELISA). The results showed that the SH-SAW biosensor achieved a high correlation coefficient (R = 0.9997) with different concentrations (34.375–1100 ng/mL) of the “spike-in” anti-N protein antibodies. Compared to ELISA, the SH-SAW biosensor has better sensitivity and can detect anti-N protein IgG signals earlier than ELISA on day 6 ( p < 0.05). Overall, in this study, we demonstrated that the SH-SAW biosensor is a promising platform for rapid in vitro diagnostic (IVD) testing, especially for antigen or antibody testing.
【저자키워드】 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), surface acoustic wave (SAW), in vitro diagnostic (IVD), 【초록키워드】 COVID-19, antibodies, SARS-CoV-2, IgG, Coronavirus disease 2019, Antibody testing, coronavirus, pandemic, antibody, diagnostic, in vitro, anti-SARS-CoV-2, ELISA, Antigen, Protein, immunoassay, sensitivity, outbreak, nucleocapsid, nucleic acid test, sera, SARS-CoV-2 N protein, viral culture, pathogen detection, platform, Concentration, acute respiratory syndrome, high correlation, injection, IVD, enzyme-linked immunosorbent, detect, collected, develop, evaluated, demonstrated, 【제목키워드】 IgG, Test, Rapid, surface, novel, Wave, shear, Acoustic,