SARS-CoV-2 viral antigen detection may be an interesting alternative to RT-PCR for the diagnosis of SARS-CoV-2 infection as a less laborious or expensive method but requires validation. This study aimed to compare the performance of the DiaSorin™ LiaisonXL automated quantitative antigen test (QAT) and the AAZ™ rapid antigen test (RAT) to the DiaSorin™ MDX RT-PCR assay. A total of 242 nasopharyngeal samples were tested at La Pitié-Salpêtrière University Hospital (Paris, France). Performances for the detection of variants of SARS-CoV-2 were further investigated. RATs were visually read for qualitative results and band intensity was determined. Overall sensitivity was 63.2% for QAT and 58.6% for RAT. For RT-PCR Ct value 25, sensitivity was 89.8% for both tests. Both tests showed comparable sensitivity for detection of variants. There was a strong relationship between antigen concentration and band positivity. On the same set of samples these tests share similar performances.
【저자키워드】 COVID-19, SARS-CoV-2, diagnostic, Nasopharyngeal swab, nucleocapsid, antigen testing, manual assay, automated assay, 【초록키워드】 Infection, RT-PCR, variants, Antigen, sensitivity, France, automated, antigen detection, performance, university, Quantitative, RT-PCR assay, Concentration, RAT, intensity, Paris, RT-PCR Ct, variants of SARS-CoV-2, nasopharyngeal sample, SARS-CoV-2 viral, tested, investigated, was determined, less, comparable, diagnosis of SARS-CoV-2, 【제목키워드】 Test, detection, Rapid,