While some individuals infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) present mild-to-severe disease, many SARS-CoV-2-infected individuals are asymptomatic. We sought to identify the distinction of immune response between asymptomatic and moderate patients. We performed single-cell transcriptome and T-cell/B-cell receptor (TCR/BCR) sequencing in 37 longitudinal collected peripheral blood mononuclear cell samples from asymptomatic, moderate, and severe patients with healthy controls. Asymptomatic patients displayed increased CD56 bri CD16 − natural killer (NK) cells and upregulation of interferon-gamma in effector CD4 + and CD8 + T cells and NK cells. They showed more robust TCR clonal expansion, especially in effector CD4 + T cells, but lack strong BCR clonal expansion compared to moderate patients. Moreover, asymptomatic patients have lower interferon-stimulated genes (ISGs) expression in general but large interpatient variability, whereas moderate patients showed various magnitude and temporal dynamics of the ISGs expression across multiple cell populations but lower than a patient with severe disease. Our data provide evidence of different immune signatures to SARS-CoV-2 in asymptomatic infections.
【저자키워드】 Infectious diseases, Infection, 【초록키워드】 Transcriptome, SARS-CoV-2, coronavirus, immune response, T cells, Sequencing, interferon, NK cells, severe acute respiratory syndrome Coronavirus, CD4, CD8, Peripheral blood, asymptomatic infections, T cell, Asymptomatic, Patient, receptor, Severe patient, respiratory, temporal dynamics, TCR, disease, expression, moderate, asymptomatic patients, patients, single-cell, asymptomatic patient, ISGs, ISG, BCR, Interferon-gamma, CD16, Evidence, interferon-stimulated genes, severe disease, evidence of, clonal expansion, mononuclear cell, natural killer, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, infected individuals, individual, Variability, upregulation, healthy controls, CD56, while, interferon-stimulated gene, cell population, Cell, robust, immune signature, identify, performed, lack, collected, magnitude, SARS-CoV-2-infected individual, 【제목키워드】 immune profiling, Patient, Asymptomatic COVID-19, reveal,