We present supplementary data for the published article, “Hitting the diagnostic sweet spot: Point-of-care SARS-CoV-2 salivary antigen testing with an off-the-shelf glucometer” [1] . The assay described is designed to be performed at home or in a clinic without expensive instrumentation or professional training. SARS-CoV-2 is detected by an aptamer-based assay that targets the Nucleocapsid (N) or Spike (S) antigens. Binding of the N or S protein to their respective aptamer results in the competitive release of a complementary antisense-invertase enzyme complex. The released enzyme then catalyzes the conversion of sucrose to glucose that is measured by an off-the-shelf glucometer. The data presented here describe the optimization of the assay parameters and their contribution to developing this aptamer-based assay to detect SARS-CoV-2. The assay performance was checked in a standard buffer, contrived samples, and patient samples validated with well-established scientific methods. The resulting dataset can be used to further develop glucometer-based assays for diagnosing other communicable and non-communicable diseases.
【저자키워드】 COVID-19, SARS-CoV-2, Population screening, aptamer, point-of-care, enzyme, Glucometer, 【초록키워드】 S protein, spike, diagnostic, Antigen, Patient, antigens, target, dataset, communicable, non-communicable diseases, Glucose, complementary, complex, diagnosing, parameter, buffer, salivary, sweet, resulting, described, performed, detect, develop, can be used, released, checked, 【제목키워드】 SARS-CoV-2 detection,