Aim: In the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), reverse transcriptase-PCR (RT-PCR) technique is often used. We evaluated the compatibility of SARS-CoV-2 RT-PCR kits containing different gene targets during the pandemic. Materials & methods: Samples were tested by Bio-Speddy ® ( RdRp gene) and Diagnovital ® ( RdRp + E genes). The correlation between two assays were determined by Deming regression analysis and chi-square analyses. Results: Diagnovital PCR kit showed amplification in a narrow Ct range and conveniently sharper exponential amplification curves than Bio-Speedy PCR kit. While the correlation between the findings of the two kits was apparent even with single gene target, this correlation increased when a secondary biomarker was added to the correlation calculations. Conclusion: We have observed high correlation between different PCR kits, however, using different PCR kits during the pandemic may provide a more accurate diagnosis of SARS-CoV-2, since despite correlation there are a number of patients showing contradicting diagnosis.
【저자키워드】 COVID-19, SARS-CoV-2, coronavirus, RT-PCR, 【초록키워드】 pandemic, Biomarker, Diagnosis, amplification, PCR, Patient, SARS-CoV-2 RT-PCR, correlation, RdRp gene, regression analysis, acute respiratory syndrome, high correlation, material, single gene, while, Genes, amplification curve, gene target, tested, Sample, evaluated, added, analyses, diagnosis of SARS-CoV-2, 【제목키워드】 coronavirus disease, pandemic, investigation, acute respiratory syndrome, gene target,