SARS-CoV-2 infection has recently been declared a pandemic. Some patients showing severe symptoms exhibit drastic inflammation and airway damage. In this study, we re-analyzed published scRNA-seq data of COVID-19 patient bronchoalveolar lavage fluid to further classify and compare immunological features according to the patient’s disease severity. Patients with severe symptoms showed DNA damage and apoptotic features of epithelial cells. Our results suggested that epithelial damage was associated with neutrophil infiltration. Myeloid cells of severe patients showed higher expression of proinflammatory cytokines and chemokines such as CXCL8. As a result, neutrophils were abundant in lungs of patients from the severe group. Furthermore, recruited neutrophils highly expressed genes related to neutrophil extracellular traps. Neutrophil-mediated inflammation was regulated by glucocorticoid receptor expression and activity. Based on these results, we suggest that severe COVID-19 symptoms may be determined by differential expression of glucocorticoid receptors and neutrophils.
【저자키워드】 COVID-19, SARS-CoV-2, neutrophil, glucocorticoid, BAL, scRNA-seq, CXCL8, 【초록키워드】 Neutrophils, Inflammation, pandemic, Neutrophil extracellular traps, SARS-COV-2 infection, disease severity, lung, chemokine, airway, DNA damage, Bronchoalveolar lavage fluid, Patient, epithelial cells, receptor, Severe patient, expression, epithelial, myeloid, COVID-19 patient, Proinflammatory cytokine, differential expression, severe group, scRNA-seq data, neutrophil infiltration, apoptotic, immunological, feature, Cell, recruited, suggested, regulated, severe symptom, drastic, expressed gene, severe COVID-19 symptom, 【제목키워드】 axis,