Summary Background As coronavirus disease 2019 caused by severe acute respiratory syndrome coronavirus 2 evolved only recently, the persistency of the anti-viral antibody response remains to be determined. Methods We prospectively followed 29 coronavirus disease 2019 cases, mean age 44 ± 13.2 years. Except for one participant with a pre-existing diagnosis of rheumatoid arthritis, all other participants were previously healthy. We determined anti-viral binding antibodies at 2–10 weeks, 3 months, and 6 months after disease onset as well as neutralizing antibodies at 6 months. Two binding antibody assays were used, targeting the S1 subunit of the spike protein, and the receptor binding domain. Results All participants fully recovered spontaneously except for one who had persisting hyposmia. Antibodies to the receptor binding domain persisted for 6 months in all cases with a slight increase of titers, whereas antibodies to S1 dropped below the cut-off point in 2 participants and showed a minimal decrease on average, mainly at month 3 of follow-up in males; however, neutralizing antibodies were detected in all samples at 6 months of follow-up. Conclusion There is a stable and persisting antibody response against acute respiratory syndrome coronavirus 2 at 6 months after infection. Neutralizing antibodies confirm virus specificity. As the number of coronavirus disease 2019 convalescent cases is increasing sharply, antibody testing should be implemented to identify immunized individuals. This information can be helpful in various settings of professional and private life.
【저자키워드】 Immunity, virus, ELISA, Prospective, Neutralizing, 【초록키워드】 coronavirus disease, neutralizing antibody, Arthritis, Coronavirus disease 2019, Antibody testing, coronavirus, Neutralizing antibodies, antibody, Infection, Diagnosis, severe acute respiratory syndrome Coronavirus, rheumatoid arthritis, Spike protein, Receptor binding domain, Anti-viral, specificity, age, Follow-up, respiratory, convalescent, information, disease, Hyposmia, S1 subunit, disease onset, titers, binding antibody, life, except for, cut-off point, acute respiratory syndrome, acute respiratory syndrome coronavirus, All participants, average, participant, cut-off, anti-viral antibody, decrease, immunized, Result, the S1 subunit, identify, caused, healthy, were used, the spike protein, individuals, All participant, antibody response against, dropped, 【제목키워드】 SARS-CoV-2 antibody, COVID-19 cohort,