The duration of humoral and cellular immune memory following SARS-CoV-2 infection in populations in least developed countries remains understudied but is key to overcome the current SARS-CoV-2 pandemic. Sixty-four Cambodian individuals with laboratory-confirmed infection with asymptomatic or mild/moderate clinical presentation were evaluated for Spike (S)-binding and neutralizing antibodies and antibody effector functions during acute phase of infection and at 6-9 months follow-up. Antigen-specific B cells, CD4 + and CD8 + T cells were characterized, and T cells were interrogated for functionality at late convalescence. Anti-S antibody titers decreased over time, but effector functions mediated by S-specific antibodies remained stable. S- and nucleocapsid (N)-specific B cells could be detected in late convalescence in the activated memory B cell compartment and are mostly IgG + . CD4 + and CD8 + T cell immune memory was maintained to S and membrane (M) protein. Asymptomatic infection resulted in decreased antibody-dependent cellular cytotoxicity (ADCC) and frequency of SARS-CoV-2-specific CD4 + T cells at late convalescence. Whereas anti-S antibodies correlated with S-specific B cells, there was no correlation between T cell response and humoral immune memory. Hence, all aspects of a protective immune response are maintained up to nine months after SARS-CoV-2 infection and in the absence of re-infection.
【저자키워드】 SARS-CoV-2, T cell immunity, B cell immunity, antibody effector function, long term immune response, 【초록키워드】 neutralizing antibody, IgG, spike, antibody, SARS-COV-2 infection, SARS-CoV-2 pandemic, Infection, B cells, CD4, CD8, Population, B cell, Protein, T cell, Asymptomatic, nucleocapsid, Re-infection, Antibody titer, membrane, immune memory, ADCC, Follow-up, T cell response, function, antibody-dependent cellular cytotoxicity, cellular, Frequency, humoral, acute phase, individual, protective immune response, no correlation, laboratory-confirmed, anti-S antibody, country, Cell, memory B, remained, evaluated, nine, characterized, activated, absence, overcome, correlated, S-specific antibody, 【제목키워드】 longitudinal, Asian, Month,