A high-throughput mass spectrometry based M pro assay identifies penicillin esters as new SARS-CoV-2 M pro inhibitors. The main viral protease (M pro ) of SARS-CoV-2 is a nucleophilic cysteine hydrolase and a current target for anti-viral chemotherapy. We describe a high-throughput solid phase extraction coupled to mass spectrometry M pro assay. The results reveal some β-lactams, including penicillin esters, are active site reacting M pro inhibitors, thus highlighting the potential of acylating agents for M pro inhibition.
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【초록키워드】 SARS-CoV-2, protease, inhibitors, Anti-viral, Chemotherapy, cysteine, M pro, new SARS-CoV-2, identify, highlighting, nucleophilic, 【제목키워드】 SARS-CoV-2, mass spectrometry, inhibitor, information, M pro, reveal, ESI,
【초록키워드】 SARS-CoV-2, protease, inhibitors, Anti-viral, Chemotherapy, cysteine, M pro, new SARS-CoV-2, identify, highlighting, nucleophilic, 【제목키워드】 SARS-CoV-2, mass spectrometry, inhibitor, information, M pro, reveal, ESI,