This study aimed to investigate the environmental contamination of nucleic acid at 2019 novel coronavirus (2019-nCOV) vaccination site and to evaluate the effect of improvement to the vaccination process. Nucleic acid samples were collected from the surface of the objects in 2019-nCOV vaccination point A (used between 15 November 2020 and 25 December 2020) and point B (used after 27 December 2020) in a comprehensive tertiary hospital. Samples were collected from point A before improvement to the vaccination process, and from point B (B 1 and B 2 ) after improvement to the vaccination process. The real-time fluorescence polymerase chain reaction method was used for detection. The positive rate of vaccination room was 47.06% (24/51) at point A. No positive result was found in point B 1 both at working hours (0/27) and after terminal disinfection (0/27). In point B 2 , the positive results were found in vaccine’s outer packaging and staff gloves at working hours, with a positive rate of 7.41% (2/27). The positive rate was 0 (0/27) after terminal disinfection in point B 2 . The nucleic acid contamination in the vaccination room of 2019-nCOV vaccine nucleic acid sampling point is serious, which can be avoided through the improvement and intervention (such as personal protection, vaccination operation and disinfection methods).
【저자키워드】 vaccination, 2019-nCoV, Pollution, 【초록키워드】 Vaccine, hospital, Intervention, 2019 novel coronavirus, nucleic acid, Contamination, positive rate, positive result, polymerase chain, was used, collected, Sample, evaluate the effect, 【제목키워드】 nucleic acid, Contamination, Can,