Abstract The pandemic of COVID‐19 has caused enormous fatalities worldwide. Serological assays are important for detection of asymptomatic or mild cases of COVID‐19, and sero‐prevalence and vaccine efficacy studies. Here, we evaluated and compared the performance of seven commercially available enzyme‐linked immunosorbent assay (ELISA)s for detection of anti‐severe acute respiratory syndrome corona virus 2 (SARS‐CoV‐2) immunoglobulin G (IgG). The ELISAs were evaluated with a characterized panel of 100 serum samples from qRT‐PCR confirmed COVID‐19 patients, collected 14 days post onset disease, 100 SARS‐CoV‐2 negative samples and compared the results with that of neutralization assay. Results were analysed by creating the receiver operating characteristic curve of all the assays in reference to the neutralization assay. All kits, were found to be suitable for detection of IgG against SARS‐CoV‐2 with high accuracy. The DiaPro COVID‐19 IgG ELISA showed the highest sensitivity (98%) among the kits. The assays demonstrated high sensitivity and specificity in detecting the IgG antibodies against SARS‐CoV‐2. However, the presence of IgG antibodies does not always correspond to neutralizing antibodies. Due to their good accuracy indices, these assays can also aid in tracing mild infections, in cohort studies and in pre‐vaccine evaluations.
【저자키워드】 COVID‐19, SARS‐CoV‐2, ELISA, ROC, IgG antibody assay, 【초록키워드】 IgG, Efficacy, Vaccine, pandemic, Neutralizing antibodies, virus, cohort study, COVID‐19, SARS‐CoV‐2, sensitivity, IgG antibody, Asymptomatic, Immunoglobulin, Accuracy, Sensitivity and specificity, Neutralization assay, IgG ELISA, characteristic, disease, fatality, acute respiratory syndrome, COVID‐19 patients, high accuracy, serum sample, mild infections, negative sample, Seven, Result, highest, collected, caused, evaluated, analysed, characterized, demonstrated, immunosorbent, creating, mild case, 【제목키워드】 IgG, SARS‐CoV‐2, performance, Seven, immunosorbent,