Highlights • The prevalence of infected patients with SARS-CoV-2 or other viruses is unknown. • We analyzed SARS-CoV-2 and 17 viruses in 191 patients with cold-like symptom in Japan. • 21 % of patient (n = 40) was infected at least one virus, including SARS-CoV-2 (n = 8). • No influenza virus was observed in this study. • The data implies different infectivity between influenza and SARS-CoV2. Background Severe acute respiratory coronavirus 2 (SARS-CoV-2) has spread and caused death worldwide. Preventive measures and infection control are underway, and some areas show signs of convergence. Other viruses in addition to SARS-CoV-2 cause cold-like symptoms and spread in the winter. However, the extent to which SARS-CoV-2, influenza viruses and other causative viruses have prevailed since implementing preventive measures is unclear. Objectives We aim to investigate the incidence of causative viruses and pathogens in patients. Study design We collected 191 nasopharyngeal swabs from patients with cold-like symptoms in Japan. All samples were subjected to multiplex PCR with the FilmArray Respiratory Panel and reverse transcription PCR (RT-PCR) to detect SARS-CoV-2. Results FilmArray Respiratory Panel analysis detected at least one virus in 32 of 191 patients with cold-like symptoms (21 %). Of these, we frequently identified human rhinoviruses/enteroviruses (5.8 %, n=11), human metapneumovirus (3.7 %, n=7), coronavirus 229E (2.1 %, n=4) and coronavirus OC43 (1.6 %, n=3); while no influenza viruses were detected. RT-PCR analysis detected SARS-CoV-2 (4.2 %, n=8) in patients who were not infected with the aforementioned respiratory viruses. Conclusions Co-infection with SARS-CoV-2 and other viruses was not observed. Causative viruses remain prevalent after implementing preventive measures. SARS-CoV-2 differs from influenza viruses in its infectivity.
【저자키워드】 COVID-19, SARS-CoV-2, Influenza, Infection, PCR, FilmArray, 【초록키워드】 infection control, coronavirus, SARS-CoV2, Multiplex PCR, Influenza virus, Symptom, RT-PCR, virus, respiratory viruses, Spread, Prevalence, Nasopharyngeal swab, pathogen, Patient, death, Japan, preventive measures, incidence, respiratory, reverse transcription PCR, Other, patients, OC43, preventive measure, RT-PCR analysis, métapneumovirus, coronavirus 229E, objective, prevalent, Respiratory Coronavirus, Panel analysis, Result, analyzed, detect, collected, caused, addition, infected patient, Causative, cold-like symptom, Panel, 【제목키워드】 viruses, Japan, influenza viruses, Intensive, preventive measure, determine,