Emerging life threatening pathogens such as severe acute aspiratory syndrome-coronavirus (SARS-CoV), avian-origin influenzas H7N9, and the Middle East respiratory syndrome coronavirus (MERS-CoV) have caused a high case-fatality rate and psychological effects on society and the economy. Therefore, a simple, rapid, and safe method to investigate a therapeutic approach against these pathogens is required. In this study, a simple, quick, and safe cell adhesion inhibition assay was developed to determine the potential cellular binding site on the SARS-CoV spike protein. Various synthetic peptides covering the potential binding site helped to minimize further the binding motif to 10–25 residues. Following analyses, 2 peptides spanning the 436–445 and 437–461 amino acids of the spike protein were identified as peptide inhibitor or peptide vaccine candidates against SARS-CoV.
【저자키워드】 SARS-CoV, ACE2, angiotensin-converting enzyme 2, RBD, receptor-binding domain, Spike protein, VERO E6 cells, S, spike, RBM, receptor-binding motif, SARS-CoV, severe acute respiratory syndrome-coronavirus, MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Binding motif, 【초록키워드】 SARS-CoV, Influenza, peptide, MERS-CoV, binding site, pathogen, vaccine candidate, coronavirus spike protein, Psychological, binding, Amino acid, cellular, Therapeutic approach, Safe, Middle East, H7N9, acute respiratory syndrome, SARS-CoV spike protein, residues, respiratory syndrome coronavirus, motif, Effect, approach, Cell, synthetic peptide, Peptide inhibitor, caused, required, determine, the spike protein, against SARS-CoV, analyses, life threatening,