The SARS coronavirus (SARS-CoV) has the potential to cause serious disease that can spread rapidly around the world. Much of our understanding of SARS-CoV pathogenesis comes from in vitro experiments. Unfortunately, in vitro experiments cannot replicate all the complexity of the in vivo infection. For example, proteases in the respiratory tract cleave the SARS-CoV surface protein to facilitate viral entry, but these proteases are not present in vitro. Unfortunately, proteases might also have an effect on other parts of the replication cycle. Here, we use mathematical modeling to estimate parameters characterizing viral replication for SARS-CoV in the presence of trypsin or elastase, and in the absence of either. In addition to increasing the infection rate, the addition of trypsin and elastase causes lengthening of the eclipse phase duration and the infectious cell lifespan.
【저자키워드】 coronavirus, protease, mathematical model, Parameter estimation, In vitro infections, 【초록키워드】 Pathogenesis, SARS-CoV, Infection, in vitro, viral entry, Replication, Spread, viral replication, respiratory tract, infection rate, trypsin, SARS Coronavirus, in vivo, disease, parameter, surface protein, Cell, example, addition, replicate, facilitate, absence, cause, in vitro experiments, mathematical, cleave, in vitro experiment, 【제목키워드】 in vitro, trypsin, SARS-CoV infection,