The virucidal activities were evaluated by spraying slightly acidic hypochlorous acid waters (SAHWs) containing various concentrations of free available chlorine – 100, 200, 300 and 500 ppm (SAHW-100, -200, -300 and -500, respectively) – toward aerosol of an avian coronavirus (infectious bronchitis virus: IBV). The viral solution was supplemented with 0.5% fetal bovine serum (FBS) to simulate normal human droplets generated by sneezing or coughing in a real-life scenario. The virus containing 0.5% FBS was sprayed and exposed to SAHWs for a few seconds in a closed chamber, before reaching the air sampler. The results showed that IBV exposed to SAHW-100 and -200 for a few seconds decreased by 0.21 log 10 and 0.80 log 10 , respectively, compared to the pre-exposed samples to SAHWs as controls. On the other hand, reductions of 1.16 log 10 and 1.67 log 10 were achieved following the exposure to SAHW-300 and -500, respectively, within a few seconds. These results suggest that SAHWs have rapid in vitro virucidal activity toward aerosolized IBV. The findings obtained for IBV might basically be applicable in relation to SARS-CoV-2, given the resemblance between the two viruses. To prevent human-to-human transmissions by aerosols, the inactivation of viruses in the air by exposure to SAHWs for a few seconds seems to be an effective way.
【저자키워드】 coronavirus, aerosol, Disinfection, Infectious Bronchitis Virus, spray, SAHW (slightly acidic hypochlorous acid water), 【초록키워드】 viruses, SARS-CoV-2, droplet, in vitro, virus, Aerosols, activity, serum, inactivation, virucidal, bovine, Concentration, reduction, bronchitis, Acidic, human-to-human transmission, avian coronavirus, coughing, IBV, Prevent, controls, effective, log, evaluated, fetal, available chlorine, FBS, 【제목키워드】 droplet, in vitro, virucidal, Rapid, Acidic,