SARS-CoV-2 provokes a robust T cell response. Peptide-based studies exclude antigen processing and presentation biology, which may influence T cell detection studies. To focus on responses to whole virus and complex antigens, we used intact SARS-CoV-2 and full-length proteins with DCs to activate CD8 and CD4 T cells from convalescent people. T cell receptor (TCR) sequencing showed partial repertoire preservation after expansion. Resultant CD8 T cells recognize SARS-CoV-2–infected respiratory tract cells, and CD4 T cells detect inactivated whole viral antigen. Specificity scans with proteome-covering protein/peptide arrays show that CD8 T cells are oligospecific per subject and that CD4 T cell breadth is higher. Some CD4 T cell lines enriched using SARS-CoV-2 cross-recognize whole seasonal coronavirus (sCoV) antigens, with protein, peptide, and HLA restriction validation. Conversely, recognition of some epitopes is eliminated for SARS-CoV-2 variants, including spike (S) epitopes in the Alpha, Beta, Gamma, and Delta variant lineages.
【저자키워드】 T cells, Infectious disease, dendritic cells, antigen-presenting cells, 【초록키워드】 SARS-CoV-2, coronavirus, Sequencing, peptide, delta variant, virus, CD4, CD8, Protein, T cell, SARS-CoV-2 variants, response, Gamma, antigens, Beta, HLA, convalescent, epitope, TCR, T cell receptor, lineages, T cell response, inactivated, breadth, CD8 T cell, CD4 T cell, Viral antigen, subject, complex, respiratory tract cells, antigen processing, robust, full-length protein, detect, recognize, activate, eliminated, provoke, 【제목키워드】 SARS-CoV-2, coronavirus, T cell response, cross-reactive, component, include,