Highlights • HCoV−OC43 is involved in healthcare–associated infections. • HCoV−OC43 genotypes B, E, F and G are identified. • Asian and European strains of HCoV−OC43 circulate among patients. Background While respiratory viral infections are recognized as a frequent cause of illness in hematopoietic stem cell transplantation (HSCT) recipients, HCoV−OC43 infections have rarely been investigated as healthcare-associated infections in this population. Objectives In this report, HCoV−OC43 isolates collected from HSCT patients were retrospectively characterized to identify potential clusters of infection that may stand for a hospital transmission. Study design Whole-genome and S gene sequences were obtained from nasal swabs using next-generation sequencing and phylogenetic trees were constructed. Similar identity matrix and determination of the most common ancestor were used to compare clusters of patient’s sequences. Amino acids substitutions were analysed. Results Genotypes B, E, F and G were identified. Two clusters of patients were defined from chronological data and phylogenetic trees. Analyses of amino acids substitutions of the S protein sequences identified substitutions specific for genotype F strains circulating among European people. Conclusions HCoV−OC43 may be implicated in healthcare-associated infections.
【저자키워드】 Whole genome sequencing, phylogenetic analyses, Hematopoietic cell transplant, Care-associated infections, human coronavirus, 【초록키워드】 hospital, Infection, Transmission, infections, Next-generation sequencing, Patient, Genotype, Cluster, nasal swab, patients, respiratory viral infection, Amino acid, S gene, Phylogenetic tree, strain, Phylogenetic, HSCT, Stem cell transplantation, identity, Asian, recipients, sequence, Substitution, circulating, while, isolate, objective, European, Result, defined, identify, collected, involved, investigated, analysed, characterized, were used, the S protein, implicated, HSCT patient, 【제목키워드】 whole-genome sequencing, Infection, HCoV-OC43, molecular, Cell,