[저자] Joshua M. Francis, Del Leistritz-Edwards, Augustine Dunn, Christina Tarr, Jesse Lehman, Conor Dempsey, Andrew Hamel, Violeta Rayon, Gang Liu, Yuntong Wang, Marcos Wille, Melissa Durkin, Kane Hadley, Aswathy Sheena, Benjamin Roscoe, Mark Ng, Graham Rockwell, Margaret Manto, Elizabeth Gienger, Joshua Nickerson,
MGH COVID-19 Collection and Processing Team2‡
, Amir Moarefi, Michael Noble, Thomas Malia, Philip D. Bardwell, William Gordon, Joanna Swain, Mojca Skoberne, Karsten Sauer, Tim Harris, Ananda W. Goldrath, Alex K. Shalek, Anthony J. Coyle, Christophe Benoist, Daniel C. Pregibon, Nikolaus Jilg, Jonathan Li, Alex Rosenthal, Colline Wong, George Daley, David Golan, Howard Heller, Arlene Sharpe, Betelihem A. Abayneh, Patrick Allen, Diane Antille, Katrina Armstrong, Siobhan Boyce, Joan Braley, Karen Branch, Katherine Broderick, Julia Carney, Andrew Chan, Susan Davidson, Michael Dougan, David Drew, Ashley Elliman, Keith Flaherty, Jeanne Flannery, Pamela Forde, Elise Gettings, Amanda Griffin, Sheila Grimmel, Kathleen Grinke, Kathryn Hall, Meg Healy, Deborah Henault, Grace Holland, Chantal Kayitesi, Vlasta LaValle, Yuting Lu, Sarah Luthern, Jordan Marchewka Schneider, Brittani Martino, Roseann McNamara, Christian Nambu, Susan Nelson, Marjorie Noone, Christine Ommerborn, Lois Chris Pacheco, Nicole Phan, Falisha A Porto, Edward Ryan, Kathleen Selleck, Sue Slaughenhaupt, Kimberly Smith Sheppard, Elizabeth Suschana, Vivine Wilson, Mary Carrington, Maureen Martin, Yuko Yuki, Galit Alter, Alejandro Balazs, Julia Bals, Max Barbash, Yannic Bartsch, Julie Boucau, Mary Carrington, Josh Chevalier, Fatema Chowdhury, Edward DeMers, Kevin Einkauf, Jon Fallon, Liz Fedirko, Kelsey Finn, Pilar Garcia-Broncano, Musie S. Ghebremichael, Ciputra Hartana, Chenyang Jiang, Kelly Judge, Paulina Kaplonek, Marshall Karpell, Peggy Lai, Evan C. Lam, Kristina Lefteri, Xiaodong Lian, Mathias Lichterfeld, Daniel Lingwood, Hang Liu, Jinqing Liu, Natasha Ly, Zachary Manickas Hill, Ashlin Michell, Ilan Millstrom, Noah Miranda, Claire O'Callaghan, Matthew Osborn, Shiv Pillai, Yelizaveta Rassadkina, Alexandra Reissis, Francis Ruzicka, Kyra Seiger, Libera Sessa, Christianne Sharr, Sally Shin, Nishant Singh, Weiwei Sun, Xiaoming Sun, Hannah Ticheli, Alicja Trocha-Piechocka, Bruce Walker, Daniel Worrall, Xu G. Yu, Alex Zhu
[Category] COVID-19,
[Article Type] Research Articles
[Source] PMC
Description Effective presentation of antigens by HLA class I molecules to CD8 + T cells is required for viral elimination and generation of long-term immunological memory. In this study, we applied a single-cell, multi-omic technology to generate a unified ex vivo characterization of the CD8 + T cell response to SARS-CoV-2 across 4 major HLA class I alleles. We found that HLA genotype conditions key features of epitope specificity, TCR α/β sequence diversity, and the utilization of pre-existing SARS-CoV-2 reactive memory T cell pools. Single-cell transcriptomics revealed functionally diverse T cell phenotypes of SARS-CoV-2-reactive T cells, associated with both disease stage and epitope specificity. Our results show that HLA variations significantly influence the CD8 + T cell repertoire shape and utilization of immune recall upon SARS-CoV-2 infection. We perform a unified characterization of CD8 + T cell responses to SARS-CoV-2, revealing pre-existing reactivity shaped by HLA genotype.
All Keywords
【초록키워드】 SARS-CoV-2, Single-cell transcriptomics, T cells, SARS-COV-2 infection, alleles, CD8, Immunological memory, immune, Antigen, specificity, T cell, Genotype, HLA, recall, epitope, TCR, single-cell, T cell response, HLA class I, Ex vivo, disease stage, sequence, T cell phenotype, description, HLA variation, memory T, feature, Cell, reactive, significantly, required, generate, applied, condition, reactivity, 【제목키워드】 SARS-CoV-2, Variation, CD8, T cell, HLA, memory response, cross-reactive, determine,