Highlights • Polyethylene glycol (PEG)-mediated precipitation is an established methodology for concentration of virus stocks and is applied here to SARS-COV-2 derived from VeroE6 supernatant. • PEG precipitation resulted in a 1.5log10 increase in SARS-CoV-2 infectious titres in VeroE6, Calu-3 and Caco-2 cells. • This protocol is a simple and reliable methodology for the concentration of SARS-COV-2 stocks for a range of virology applications. The development of medical countermeasures against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires robust viral assays. Here we have adapted a protocol for polyethylene glycol (PEG)-mediated precipitation of SARS-CoV-2 stocks without the need for ultracentrifugation. Virus precipitation resulted in a ∼1.5 log 10 increase in SARS-CoV-2 titres of virus prepared in VeroE6 cells and enabled the infection of several immortalized human cell lines (Caco-2 and Calu-3) at a high multiplicity of infection not practically achievable without virus concentration. This protocol underscores the utility of PEG-mediated precipitation for SARS-CoV-2 and provides a resource for a range of coronavirus research areas.
【저자키워드】 COVID-19, SARS-CoV-2, virus concentration, PEG precipitation, 【초록키워드】 coronavirus, protocol, Infection, virus, Research, methodology, utility, resource, Calu-3, Caco-2 cells, Concentration, Polyethylene glycol, Caco-2, glycol, multiplicity of infection, acute respiratory syndrome, titre, ultracentrifugation, supernatant, robust, log, assays, applied, provide, increase in, virus stock, human cell line, immortalized, medical countermeasure, VeroE6 cell, 【제목키워드】 glycol,