The use of monoclonal neutralizing antibodies (mNAbs) is being actively pursued as a viable intervention for the treatment of Severe Acute Respiratory Syndrome CoV-2 (SARS-CoV-2) infection and associated coronavirus disease 2019 (COVID-19). While highly potent mNAbs have great therapeutic potential, the ability of the virus to mutate and escape recognition and neutralization of mNAbs represents a potential problem in their use for the therapeutic management of SARS-CoV-2. Studies investigating natural or mNAb-induced antigenic variability in the receptor binding domain (RBD) of SARS-CoV-2 Spike (S) glycoprotein, and their effects on viral fitness are still rudimentary. In this manuscript we described experimental approaches for the selection, identification, and characterization of SARS-CoV-2 monoclonal antibody resistant mutants (MARMs) in cultured cells. The ability to study SARS-CoV-2 antigenic drift under selective immune pressure by mNAbs is important for the optimal implementation of mNAbs for the therapeutic management of COVID-19. This will help to identify essential amino acid residues in the viral S glycoprotein required for mNAb-mediated inhibition of viral infection, to predict potential natural drift variants that could emerge upon implementation of therapeutic mNAbs, as well as vaccine prophylactic treatments for SARS-CoV-2 infection. Additionally, it will also enable the assessment of MARM viral fitness and its potential to induce severe infection and associated COVID-19 disease.
【저자키워드】 COVID-19, SARS-CoV-2, Neutralizing antibodies, spike glycoprotein, monoclonal antibodies, RBD, Monoclonal antibody resistant mutant, Viral drift, 【초록키워드】 Treatment, coronavirus disease, neutralizing antibody, viral infection, Severe infection, Vaccine, spike, neutralization, SARS-COV-2 infection, monoclonal antibody, variant, Infection, Intervention, virus, Receptor binding domain, COVID-19 disease, management, implementation, therapeutic, glycoprotein, respiratory, predict, monoclonal, S glycoprotein, antigenic, Variability, therapeutic potential, cultured cells, help, immune pressure, viral fitness, manuscript, selective, prophylactic treatment, while, amino acid residue, Effect, approach, described, identify, required, induce, mutate, resistant mutant, 【제목키워드】 monoclonal antibody, Selection, resistant mutant,