Abstract
Background: Data regarding humoral and cellular response against SARS-CoV-2 in children are scarce. We analysed seroconversion rate, decrease of anti-RBD IgG antibodies over time and T-cell response in paediatric patients who suffered COVID-19.
Methods: Longitudinal study of paediatric patients COVID-19 diagnosed by positive molecular assay in nasopharyngeal swabs. Blood samples were drawn 1-2 months and 6-7 months after acute infection. Anti-RBD IgG were determined using the Alinity® SARS-CoV-2 IgG II Quant assay (Abbott). Cellular immune response was analysed by T-SPOT® SARS-CoV-2 assay kit (Oxford Immunotec Ltd.).
Results: 27/39 (69,2%) patients seroconverted. Despite a significant decrease in antibody levels over time (p < 0,01), no children seroreverted between first and second visits. Only 6/16 (37,2%) children under 6 years-old were seropositive compared to 21/23 (91,3%) over 6 years-old (p < 0,01). Highest antibody levels were found in seropositive younger children (p = 0,036). Thirteen (33,3%) children showed T-cell response. Among participants showing humoral response, no cellular response was detected in 14 (51,9%).
Conclusions: Anti-RBD IgG antibodies persistence at 6-7-months after SARS-CoV-2 infection was observed. A different IgG response was found depending on age. As measured by T-SPOT, most patients did not display cellular response 6-7 months after infection.
Keywords: Cellular response; Children; Humoral response; IgG antibodies; SARS-CoV-2.
【저자키워드】 children, SARS-CoV-2., Humoral response, IgG antibodies, cellular response, 【초록키워드】 COVID-19, SARS-CoV-2, IgG, immune response, antibody, SARS-COV-2 infection, T-cell Response, Infection, IgG antibody, longitudinal study, nasopharyngeal swabs, persistence, acute infection, Patient, age, molecular, seropositive, SARS-CoV-2 IgG, humoral, Abbott, IgG response, Oxford, blood sample, participant, significant decrease, seroconversion rate, positive, decrease, diagnosed, analysed, seroconverted, suffered, anti-RBD IgG antibody, paediatric patient, 【제목키워드】 SARS-COV-2 infection, longitudinal, humoral,