Abstract
Purpose: The accuracy of level of anti- severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies is a great concern. We aimed to compare the efficacy of anti-SARS-CoV-2 antibody detection kits from two manufacturers in evaluating the efficacy of SARS-CoV-2 vaccines.
Methods: The immune responses and consistency of four anti-SARS-CoV-2 antibodies were evaluated using two manufacturers’ antibody kits (A and B) in 61 subjects within 160 days after vaccination with the CoronaVac vaccine.
Results: The total seropositivity rates of neutralizing antibodies and IgM antibodies detected by kit A were higher than those detected by kit B (P = 0.003 and P < 0.001, respectively). Conversely, the total seropositivity rates of total antibodies and IgG antibodies were higher in kit B than kit A (P < 0.001 and P < 0.001, respectively). The consistency rates showed less than 90% agreement between the kits for the detection of the four antibodies, and the κ score showed moderate or substantial consistency. The half-lives of neutralizing antibodies, total antibodies, and IgG antibodies within 160 days after vaccination, detected by kit A were 63.88 days, 80.50 days, and 63.70 days, respectively and by kit B were 97.06 days, 65.41 days, and 77.99 days, respectively.
Conclusion: The efficacy of antibody detection differed between the two commercial anti-SARS-CoV-2 antibody kits, although there was moderate consistency, which may affect the clinical application and formulation of the vaccine strategy.
Keywords: Anti-SARS-CoV-2 antibodies; COVID-19; Consistency; SARS-CoV-2; Vaccination.
【저자키워드】 COVID-19, SARS-CoV-2, vaccination., anti-SARS-CoV-2 antibodies, Consistency, 【초록키워드】 neutralizing antibody, antibodies, Efficacy, Vaccine, coronavirus, immune response, vaccination, Neutralizing antibodies, antibody, CoronaVac, SARS-CoV-2 vaccines, IgG antibody, Antibody detection, anti-SARS-CoV-2 antibody, Accuracy, total antibody, moderate, acute respiratory syndrome, subject, IgM antibody, total antibodies, Affect, evaluated, less, the vaccine, half-live, 【제목키워드】 Efficacy, vaccination, cohort study, anti-SARS-CoV-2 antibody,