Abstract
Measurement of quantitative antibody responses are increasingly important in evaluating the immune response to infection and vaccination. In this study we describe the validation of a quantitative, multiplex serologic assay utilising an electrochemiluminescence platform, which measures IgG against the receptor binding domain (RBD), spike S1 and S2 subunits and nucleocapsid antigens of SARS-CoV-2. The assay displayed a sensitivity ranging from 73 to 91% and specificity from 90 to 96% in detecting previous infection with SARS-CoV-2 depending on antigenic target and time since infection, and this assay highly correlated with commercially available assays. The within-plate coefficient of variation ranged from 3.8-3.9% and the inter-plate coefficient of variation from 11 to 13% for each antigen.
Keywords: COVID-19; SARS-CoV-2; Serology.
【저자키워드】 COVID-19, SARS-CoV-2, Serology., 【초록키워드】 IgG, immune response, vaccination, Antibody Response, Variation, Infection, Receptor binding domain, Antigen, sensitivity, specificity, RBD, multiplex, Quantitative, platform, nucleocapsid antigen, subunit, measure, measurement, serologic assay, S1 and S2, assays, correlated, increasingly, ranged, antigenic target, infection with SARS-CoV-2, 【제목키워드】 vaccination, SARS-COV-2 infection, Multiplex assay, performance, Serologic response,