Abstract
Since late 2019, the outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the resultant spread of COVID-19 have given rise to a worldwide health crisis that is posing great challenges to public health and clinical treatment, in addition to serving as a formidable threat to the global economy. To obtain an effective tool to prevent and diagnose viral infections, we attempted to obtain human antibody fragments that can effectively neutralize viral infection and be utilized for rapid virus detection. To this end, several human monoclonal antibodies were isolated by bio-panning a phage-displayed human antibody library, Tomlinson I. The selected clones were demonstrated to bind to the S1 domain of the spike glycoprotein of SARS-CoV-2. Moreover, clone A7 in Fab and IgG formats were found to effectively neutralize the binding of S protein to angiotensin-converting enzyme 2 in the low nM range. In addition, this clone was successfully converted to quench-based fluorescent immunosensors (Quenchbodies) that allowed antigen detection within a few minutes, with the help of a handy fluorometer.
【초록키워드】 public health, SARS-CoV-2, IgG, viral infection, coronavirus, S protein, antibody, spike glycoprotein, angiotensin-converting enzyme 2, viral infections, Health, outbreak, Human monoclonal antibody, virus detection, antigen detection, diagnose, binding, Fab, Clinical treatment, acute respiratory syndrome, help, fluorescent, clone, S1 domain, Prevent, effective, neutralize, spread of COVID-19, selected, addition, demonstrated, antibody fragment, 【제목키워드】 neutralizing antibody, fluorescent, human SARS-CoV-2,