Abstract
Existing assays to measure antibody cross-reactivity against different SARS-CoV-2 spike (S) protein variants lack the discriminatory power to provide insights at the level of individual clones. Using a mass spectrometry-based approach we are able to monitor individual donors’ IgG1 clonal responses following a SARS-CoV-2 infection. We monitor the plasma clonal IgG1 profiles of 8 donors who had experienced an infection by either the wild type Wuhan Hu-1 virus or one of 3 VOCs (Alpha, Beta and Gamma). In these donors we chart the full plasma IgG1 repertoires as well as the IgG1 repertoires targeting the SARS-CoV-2 spike protein trimer VOC antigens. The plasma of each donor contains numerous anti-spike IgG1 antibodies, accounting for <0.1% up to almost 10% of all IgG1s. Some of these antibodies are VOC-specific whereas others do recognize multiple or even all VOCs. We show that in these polyclonal responses, each clone exhibits a distinct cross-reactivity and also distinct virus neutralization capacity. These observations support the need for a more personalized look at the antibody clonal responses to infectious diseases.
【초록키워드】 Diseases, antibody, VoC, SARS-COV-2 infection, variant, Infection, virus, Spike protein, cross-reactivity, Protein, response, Wuhan, VOCs, Virus neutralization, Gamma, antigens, plasma, Beta, wild type, Anti-spike, IgG1, Donor, SARS-CoV-2 spike, Support, observation, antibody cross-reactivity, profile, trimer, clones, clone, MONITOR, responses, approach, polyclonal, IgG1 antibodies, discriminatory power, lack, recognize, exhibit, individual donor, plasma IgG1, the SARS-CoV-2, 【제목키워드】 SARS-CoV-2 variant, cross-reactivity, polyclonal, IgG1 response,