Abstract
Serological assay for coronavirus 2019 (COVID-19) patients including asymptomatic cases can inform on disease progression and prognosis. A detection method taking into account multiplex, high sensitivity, and a wider detection range will help to identify and treat COVID-19. Here we integrated color-size dual-encoded beads and rolling circle amplification (RCA) into a bead-based fluorescence immunoassay implemented in a size sorting chip to achieve high-throughput and sensitive detection. We used the assay for quantifying COVID-19 antibodies against spike S1, nucleocapsid, the receptor binding domain antigens. It also detected inflammatory biomarkers including interleukin-6, interleukin-1β, procalcitonin, C-reactive protein whose concentrations range from pg mL -1 to μg mL -1 . Use of different size beads integrating with RCA results in a tunable detection range. The assay can be readily modified to simultaneously measure more COVID-19 serological molecules differing by orders of magnitude.
Keywords: COVID-19; Microfluidic Immunoassays; Multiplexing; Rolling Circle Amplification; Tunable Detection Range.
【저자키워드】 COVID-19, Multiplexing, Rolling circle amplification, Microfluidic Immunoassays, Tunable Detection Range., 【초록키워드】 Prognosis, interleukin-6, C-reactive protein, detection, procalcitonin, Receptor binding domain, Disease progression, immunoassay, sensitivity, nucleocapsid, Patient, Asymptomatic case, antigens, COVID-19 antibody, Coronavirus 2019, multiplex, serological, interleukin-1β, Inflammatory biomarker, Concentration, help, treat, RCA, identify, magnitude, Circle, Range, Rolling, 【제목키워드】 detection, Range,