Abstract
Background: Scarce information is available regarding the long-term immunogenicity of the Sputnik V vaccine. Here Sputnik V vaccinated subjects were evaluated 6 months after receiving the 2-dose prime-boost schedule.
Methods: Eighty-six hospital workers from Venezuela, 32 with a previous COVID-19 infection and 54 SARS-CoV-2 naïve subjects, were enrolled. IgG antibodies levels against the wild-type Receptor Binding Domain (RBD) were measured in an ELISA and with an in vitro ACE2-surrogate RBD binding inhibition assay at day 42 and day 180 after receiving the second dose. IgG levels were expressed in BAU/ml. Binding inhibition antibodies were expressed in IU/ml.
Results: On average, RBD-IgG levels decreased by approximately 50% between the two time-points in the COVID-19 naïve cohort (geometric mean concentration (GMC) 675 BAU/mL vs. 327 BAU/ml) and decreased by approximately 25% in the previously infected cohort (GMC 1209 BAU/mL vs 910 BAU/ml). Within our cohort, 94% showed a “good to excellent” neutralizing activity measured with the in vitro test 6 months after vaccination.
Conclusions: The Sputnik V vaccine provided long-term and durable humoral immunity in our cohort specially if a person has been both vaccinated and had a previous infection with SARS-CoV-2.
Keywords: Binding antibody units or BAU/ml; Hybrid COVID-19 immunity; IgG antibodies; International units or IU/ml; Nucleocapsid protein (NP); RBD binding inhibition assay; Receptor Binding Domain (RBD); Sputnik V vaccine.
【저자키워드】 IgG antibodies, receptor binding domain (RBD), Sputnik V vaccine, nucleocapsid protein (NP), RBD binding inhibition assay, International units or IU/ml, Hybrid COVID-19 immunity, Binding antibody units or BAU/ml, 【초록키워드】 COVID-19, SARS-CoV-2, IgG, Vaccine, vaccination, antibody, hospital, in vitro, ELISA, Protein, Humoral immunity, Neutralizing activity, Cohort, IgG antibody, COVID-19 infection, RBD, information, hybrid, Venezuela, international unit, subject, second dose, naïve subjects, average, naïve, RBD binding, geometric mean concentration, wild-type, GMC, enrolled, evaluated, provided, receiving, were expressed, were measured, antibody unit, IgG level, infection with SARS-CoV-2, 【제목키워드】 SARS-CoV-2, IgG, vaccination, Health care worker, Venezuela,